In Vitro Antiviral Testing Services
InquiryCD Formulation has accumulated rich experience in virus biology research over the years. Our in vitro antiviral testing services are based on professional virology knowledge, advanced virology technology and equipment, and are committed to providing you with accurate, fast and reliable services to help you screen antiviral drugs.
Why Do In Vitro Antiviral Testing?
Viruses are one of the main pathogens that harm human health. At present, viral diseases with high incidence rate and low cure rate such as AIDS and hepatitis C are still spreading around the world, and respiratory viruses such as influenza virus and coronavirus are constantly mutating, posing a great threat to people's life and health and social stability. Therefore, research and development of new effective antiviral drugs is crucial. In vitro antiviral testing is one of the important means to evaluate the antiviral activity of drugs and plays an important role in the development of antiviral drugs. In vitro antiviral testing use cell culture methods to observe the antiviral effects of drugs. By simulating the process of virus infection of cells in vitro, the inhibitory effect of antiviral drugs on viruses can be detected, thereby screening candidate drugs with good antiviral effects and low toxicity.
Our Method for In Vitro Antiviral Testing
The plaque reduction assay principle is based on the phenomenon that virus infection of cells leads to the formation of colorless plaques, while drugs can neutralize the virus and reduce the formation of these plaques. Through this method, the effect of different concentrations of test substances on the efficiency of virus plaque formation can be measured, and the antibody titer that neutralizes the virus can be calculated.
- Cytopathic Effect (CPE) Inhibition Assay
Cytopathic effect refers to the changes in the viability and morphology of host cells caused by viral infection. Different types of viruses can produce varying degrees of CPE in different host cells, with some rapidly destroying the entire monolayer of cells and others only affecting the morphology of some host cells. Researchers can evaluate the ability of test samples to inhibit CPE through CPE inhibition assays, allowing for the detection and screening of antiviral drugs.
Cell-based ELISA measures reduction of viral antigen in infected cells using anti-virus monoclonal antibody. The abundance of viral protein in infected cells treated with the test article compared to that of the untreated control is used as a measure of antiviral activity.
qPCR assay utilizes PCR technology to amplify viral DNA fragments and quantitatively detects viral expression levels through the intensity of fluorescent signals. Reduction of virus nucleic acid in infected cells is used an indicator of a test article’s antiviral efficacy.
- Hemagglutination-inhibition Test (HAI)
Some viral proteins, such as influenza virus lectins, have the property of binding and agglutinating red blood cells (RBCS), and this reaction can be used to qualitatively and quantitatively characterize viruses. The abundance of viral protein in infected cells treated with the test article compared to that of the untreated control is used as a measure of antiviral activity.
- Quantitative Suspension Test
During a quantitative suspension test, virus is mixed with a test solution for the desired time. The mixtures are then typically neutralized by dilution and filtration before the amount of infectious virus recovered is quantified. This data is ultimately used to determine whether the test solution has an anti-virus effect compared to the control solution.
- Antibody-dependent Enhancement (ADE) Assay
ADE effect is essentially an antibody-dependent enhancement effect, that is, when the body is infected by a pathogen, the original neutralizing antibodies not only cannot prevent the virus from invading human cells, but also enhance the replication of the virus in the body, causing serious pathological reactions. Understanding the mechanism of ADE, identifying the antigenic determinants associated with ADE in the virus, and modifying them will help to develop a more safe and effective vaccine.
We Can Perform In Vitro Antiviral Testing Against the Following Viruses
Hepatitis viruses (B and C)
Influenza virus
Herpes virus
Coxsackievirus
Respiratory syncytial virus (RSV)
Human immunodeficiency virus (HIV)
Human coronavirus NL63 (HCoV-NL63)
Human coronavirus 229E (HCoV-229E)
Enterovirus
Cytomegalovirus (CMV)
Other viruses may be available on request.
Advantages of Our In Vitro Antiviral Testing Services
- Advanced technology: We have advanced experimental equipment and professional technical team, using the most advanced cell biology, molecular biology and biochemistry technology to ensure the accuracy and reliability of experimental results.
- Rich experience: We have extensive experience in in vitro antiviral testing and have provided high-quality services to many customers. Through research on different types of viruses, we have accumulated rich data and experience and are able to provide customers with more professional advice and guidance.
- Perfect after-sales service: You can consult us anytime to get the information you want.
As an expert in the antiviral field, CD Formulation provides a wide range of antiviral services to evaluate the inhibitory activity of candidate inhibitors. If you are interested in our services, please do not hesitate to contact us for in-depth discussions.
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